Beschreibung
Inhaltsangabe1. PERSPECTIVES ON THE STATE OF INSECT TRANSGENICS. 1 David A. O'Brochta and Alfred M. Handler Introduction. 1 Transposon Vectors and Insect Transformation. 2 Transformation Markers. 4 Targeting and Stabilization. 5 Random Transgene Insertion. 5 Transgenic Strains for Biocontrol. 8 Fluorescent Protein Genetic Markers. 8 Conditional Regulation for Sterility and Lethality. 9 Paratransgenesis. 10 Viruses. 10 Perspectives on TE Spread and Genetic Manipulation for Disease Vector Control. 11 Broadening Our Perspective. 12 2. ALPHAVIRUS TRANSDUCING SYSTEMS. 19 Brian D. Foy and Ken E. Olson Introduction. 19 Alphaviruses. 20 ATS Construction. 22 Currently Used ATSs. 22 Alphavirus/Mosquito Interactions. 24 Gene Expression with ATSs. 27 ATS s for Induction of RNA Interference. 27 Biosafety Considerations for Using ATS's. 29 Conclusion. 30 3. PARATRANSGENESIS APPLIED FOR CONTROL OF TSETSE TRANSMITTED SLEEPING SICKNESS. 35 Serap Aksoy, Brian Weiss and Geoffrey Attardo Introduction. 35 Symbiosis in Tsetse. 36 Symbiont-Host Interactions During Development. 37 Tsetse's Reproductive Biology and Symbiont Transmission. 38 Biology of African Trypanosome Transmission in Tsetse. 38 Paratransgenic Gene Expression in Tsetse.
Inhaltsverzeichnis
1. PERSPECTIVES ON THE STATE OF INSECT TRANSGENICS ............ 1 David A. O¿Brochta and Alfred M. Handler Introduction ......................................................................................................................... 1 Transposon Vectors and Insect Transformation ............................................................... 2 Transformation Markers .................................................................................................... 4 Targeting and Stabilization ................................................................................................. 5 Random Transgene Insertion ............................................................................................. 5 Transgenic Strains for Biocontrol ...................................................................................... 8 Fluorescent Protein Genetic Markers ................................................................................ 8 Conditional Regulation for Sterility and Lethality .......................................................... 9 Paratransgenesis ................................................................................................................ 10 Viruses ............................................................................................................................... 10 Perspectives on TE Spread and Genetic Manipulation for Disease Vector Control ... 11 Broadening Our Perspective ............................................................................................. 12 2. ALPHAVIRUS TRANSDUCING SYSTEMS .............................................. 19 Brian D. Foy and Ken E. Olson Introduction ....................................................................................................................... 19 Alphaviruses ....................................................................................................................... 20 ATS Construction .............................................................................................................. 22 Currently Used ATSs ......................................................................................................... 22 Alphavirus/Mosquito Interactions ................................................................................... 24 Gene Expression with ATSs .............................................................................................. 27 ATS s for Induction of RNA Interference ....................................................................... 27 Biosafety Considerations for Using ATS¿s ....................................................................... 29 Conclusion .......................................................................................................................... 30 3. PARATRANSGENESIS APPLIED FOR CONTROL OF TSETSE TRANSMITTED SLEEPING SICKNESS.................. 35 Serap Aksoy, Brian Weiss and Geoffrey Attardo Introduction ....................................................................................................................... 35 Symbiosis in Tsetse ............................................................................................................ 36 Symbiont-Host Interactions During Development ......................................................... 37 Tsetse¿s Reproductive Biology and Symbiont Transmission .......................................... 38 Biology of African Trypanosome Transmission in Tsetse .............................................. 38 Paratransgenic Gene Expression in Tsetse ...................................................................... 39 Reconstitution of Tsetse with Modified Symbiont Flora .............. ...
