Artificial Assemblies with Cooperative DNA Recognition
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Springer Theses
ISBN: | 9811544255 |
---|---|
ISBN 13: | 9789811544255 |
Autor: | YU, Zutao |
Verlag: | Springer Verlag GmbH |
Umfang: | xv, 136 S., 17 s/w Illustr., 119 farbige Illustr., 136 p. 136 illus., 119 illus. in color. |
Erscheinungsdatum: | 11.05.2021 |
Auflage: | 1/2020 |
Produktform: | Kartoniert |
Einband: | KT |
This book presents three types of synthetically cooperative DNA recognizing assemblies, in order to advance the development of programmable DNA-binding pyrrole-imidazole polyamides (PIPs). PIPs represent the best-characterized class of small molecule DNA binders that can be modified to bind with any predetermined DNA sequence and regulate gene expression patterns in a transgene-free and cost-effective manner. PIPs are characterized by their small molecular size, high binding affinity, programmability, sequence selectivity, and moderate cell permeability. In recent years, there have been numerous novel studies on the applications of these biological tools; this research is thoroughly reviewed in the first chapter. There are several critical issues, however, that impede the further broad study of PIPs, which greatly concern the author. For instance, the short PIP version has an excessively hi^10 bp; this significantly decreases cell permeability. Moreover, the conventional binding strategy for PIP design cannot apply to flexible DNA binding-for example, the DNA-binding mode of a transcription factor pair. In this book, the author describes the development of three kinds of cooperative DNA-binding systems that help resolve the current highly problematic issues concerning PIPs. These three systems offer a range of significant advantages, such as favorable sequence selectivity, long recognition sequence, higher binding affinity, and a flexible gap distance. Released at a critical juncture in the application of PIPs, this book will greatly facilitate their use as therapeutic drugs in the treatment of cancer and hereditary diseases, and in regenerative medicine.
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