Antiviral Resistance in Plants

Lieferzeit: Lieferbar innerhalb 14 Tagen

106,99 

Methods and Protocols, Methods in Molecular Biology 894

ISBN: 1617798819
ISBN 13: 9781617798818
Herausgeber: John M Watson/Ming-Bo Wang
Verlag: Humana Press
Umfang: xi, 380 S., 49 s/w Illustr., 10 farbige Illustr., 380 p. 59 illus., 10 illus. in color.
Erscheinungsdatum: 12.06.2012
Auflage: 1/2012
Produktform: Gebunden/Hardback
Einband: GEB

Observing anomalies such as resistance development in plant viral infections helped in discovering double-stranded RNA. Here, skilled researchers provide reproducible, step-by-step protocols to study RNA silencing, with a focus on viral infections in plants.

Artikelnummer: 3020260 Kategorie:

Beschreibung

Inhaltsangabe1. An  Historical Overview of RNAi in Plants            John A. Lindbo 2. RNA Silencing and Antiviral Defense in Plants            Claire Agius, Andrew Eamens, Tony Millar, John M. Watson, and Ming-Bo Wang 3. Biological Chemistry of Virus-encoded Suppressors of RNA Silencing:  An Overview            Rustem T. Omarov and Herman B. Scholtof 4. Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules            Xueping Zhou and Changjun Huang 5. Strategies for Viral Cross Protection in Plants            Changyong Zhou and Yan Zhou 6. Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing            Shawn Bachan and S.P. Dinesh-Kumar 7. Virus-Induced Gene Silencing in Hexaploid Wheat Using Barley Stripe Mosaic Virus Vectors            Steven R. Scofield and Amanda S. Brandt 8. Characterization of Plant Virus-Encoded Gene Silencing Suppressors            Hajime Yaegashi, Masamichi Isogai, and Nobuyuki Yoshikawa 9. Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing            Qian-Hao Zhu and Chris Helliwell 10. Modelling Antiviral Resistance in Plants            Robert S. Anderssen and Peter M. Waterhouse 11. Isolation and Detection of Small RNAs from Plant Tissues            Neil A. Smith and Andrew L. Eamens 12. Isolation and Analysis of Small RNAs from Virus-Infected Plants            Shaun J. Curtin, Agustin Zsögön, John M. Watson, and Peter M. Waterhouse 13. Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance            Neena Mitter and Ralf G. Dietzgen 14. Artificial MicroRNAs for Plant Virus Resistance            Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs            H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases            Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis            Robert A. Owens, Teruo Sano,  and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing            Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean            Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in 'Dark Green' Islands            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy            Hajime Yaegashi, Masamichi Isogai, and Nobuyuki Yoshikawa 9. Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing            Qian-Hao Zhu and Chris Helliwell 10. Modelling Antiviral Resistance in Plants            Rober

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