Advances in the Understanding of Biological Sciences Using Next Generation Sequencing (NGS) Approaches

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106,99 

ISBN: 3319344846
ISBN 13: 9783319344843
Herausgeber: Gaurav Sablok/Sunil Kumar/Saneyoshi Ueno et al
Verlag: Springer Verlag GmbH
Umfang: xii, 241 S., 9 s/w Illustr., 31 farbige Illustr., 241 p. 40 illus., 31 illus. in color.
Erscheinungsdatum: 15.10.2016
Auflage: 1/2016
Produktform: Kartoniert
Einband: Kartoniert

This work is a compiled catalogue of such findings, where several NGS technologies ranging from the genomics, transcriptomics, metagenomics, single cell genomics, QTL, patho-genomics and patho-transcriptomics have been applied to delineate the mystery of the associated mutations, biological pathway transitions, transcriptional fluxes and patterns of host associated or adaptations to certain climatic conditions. The aims and scope of this book focuses more on the biological underpinning to initiate the cross-talks across the traits acquired or lost during the course of evolution. The structured framework of the present volume provides the applicative point of view of the NGS technologies and demonstrates the conceptual way of linking the experimentation to the NGS technologies, to aid in researchers to place their biological hypothesis in a larger context.

Artikelnummer: 9962164 Kategorie:

Beschreibung

Provides a global view of the recent advances in the biological sciences and the adaption of the pathogen to the host plants revealed using NGS. Molecular Omics is now a major driving force to learn the adaption genetics and a great challenge to the scientific community, which can be resolved through the application of the NGS technologies. The availability of complete genome sequences, the respective model species for dicot and monocot plant groups, presents a global opportunity to delineate the identification, function and the expression of the genes, to develop new tools for the identification of the new genes and pathway identification. Genome-wide research tools, resources and approaches such as data mining for structural similarities, gene expression profiling at the DNA and RNA level with rapid increase in available genome sequencing efforts, expressed sequence tags (ESTs), RNA-seq, gene expression profiling, induced deletion mutants and insertional mutants, and gene expression knock-down (gene silencing) studies with RNAi and microRNAs have become integral parts of plant molecular omics. Molecular diversity and mutational approaches present the first line of approach to unravel the genetic and molecular basis for several traits, QTL related to disease resistance, which includes host approaches to combat the pathogens and to understand the adaptation of the pathogen to the plant host. Using NGS technologies, understanding of adaptation genetics towards stress tolerance has been correlated to the epigenetics. Naturally occurring allelic variations, genome shuffling and variations induced by chemical or radiation mutagenesis are also being used in functional genomics to elucidate the pathway for the pathogen and stress tolerance and is widely illustrated in demonstrating the identification of the genes responsible for tolerance in plants, bacterial and fungal species.

Autorenporträt

Guarav Sablok, PhDResearch Fellow, Plant Functional Biology and Climate Change Cluster (C3)University of Technology, SydneyPO Box 123 Broadway NSW 2007 AustraliaT: 0039-3270484-732Email: sablokg@gmail.comSunil Kumar, M.Sc ,IPR, MCA, Ph.DSenior Scientist, Department of Bioinformatics Institute of Life Sciences Nalco Square, C S PurBhubaneswar, Odisha 275103, India T: 91-6742301500F: 91-6742300728E-mail: skybiotech@gmail.com Jimmy Kuo, Ph.DAssociate Researcher, National Museum of Marine Biology and AquariumGraduate Institute of Marine Biotechnology, National Dong Haw University2 Houwan Road, ChechengPingtung 944, TaiwanTel: 886-8-8825001 ext. 1358Fax: 886-8-8825087E-mail: jimmy@nmmba.gov.twSaneyoshi Ueno, Ph.DDepartment of Forest Genetics 1 Matsunosato, TsukubaIbaraki 3058687, JapanT: +81-29-8733211F: +81-29-8743720Email: saueno@ffpri.affrc.go.jpClaudio Varotto, Msc, Ph.DDepartment of Biodiversity and Molecular Ecology, Research and Innovation CenterFondazione Edmund Mach, Research and Innovation CenterVia E. Mach 1, 38010 San Michele allAdige (TN), ItalyT: +39 0461 615 108F: +390461650956Email: claudio.varotto@fmach.it

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