Beschreibung
This manual on "Dynamics of Membrane Proteins and Cellular Energetics" is the result of a FEBS-CNRS Course held in Grenoble and Besanc;{on in September 1987.' It appears to be, after the first, published in 1979 the fifth of the series. After focussing on the "Biochemistry of Membranes" (1979) it was the turn of "Membrane Proteins" (1981) and of Enzymes, Receptors and Carriers of Biological Membranes (1983), fo1- wed by "Membrane Proteins Isolation and Characterization" ( 1986). Al though the central issue has always been the its components and its functions, each biological membrane, manual has put the accent on somewhat different issues corresponding to the most innovative, interesting research in the field. After almost a decade this new manual appears, which stresses the aspect of the integration of membrane research at a cellular level. Such a novel emphasis is the consequence of the common interest of cell biology and biochemistry to understand the results of the biochemical analysis of membrane proteins in the context of the cell complexity. Consequently most of the experimental protocols are dealing with cellular models, but with clear reference to the function and structure of isolated membrane proteins.
Autorenporträt
InhaltsangabeOne: Theoretical Introduction.- The structure and function of Ca+2 - and phospholipid- dependent protein kinase (protein kinase C), a trans-membrane signal transducer.- Biosynthesis and mode of insertion of a stalked intrinsic membrane protein of the small intestinal brush border.- Tools and approaches to study membranes and cell organelles biogenesis.- Fluid-phase endocytosis and lysosomal enzyme excretion two facets of intracellular membrane traffic.- The neutrophil leukocyte. Properties and mechanism of activation.- Monoclonal antibodies for immunoanalysis.- Two: Experimental Section.- Experiment n° 1: Trans-membrane signaling via protein kinase C and its inhibition by staurosporine.- Experiment n° 2: Endocytosis analysis by flow cytofluorometry: study of the endocytosis of fluoresceinylated neoglycoproteins via membrane lectins of tumor cells.- Experiment n° 3: Neutrophils. Respiratory burst and exocytosis.- Experiment n°4: Endocytosis and exocytosis in Dictyostelium amoebae.- Experiment n° 5: Isolation of intact chloroplasts - Criteria of integrity.- Experiment n° 6: Methods for the isolation of higher plant vacuoles.- Experiment n° 7: Assay of in vitro microtubule assembly. Effect of microtubule associated proteins.- Experiment n° 8: Histamine release from human basophiles.- Experiment n° 9: Intracellular Ca2+ measurements in intact neutrophils.- Experiment n° 10: Synthesis and maturation of D-?-hydroxybutyrate dehydrogenase (BDH) from mitochondrial inner membrane.- Experiment n° 11: Electropermeabilization and electrofusion of cells.- Experiment n° 12: Use of monoclonal antibody against keratin in immunocytochemical techniques.
