Molecular Cloning of Glycerol-3-phosphate dehydrogenase

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Molecular cloning of salt tolerant gene from Saccharomyces cerevisiae

ISBN: 3330027266
ISBN 13: 9783330027268
Autor: Prasad, Bablu
Verlag: LAP LAMBERT Academic Publishing
Umfang: 64 S.
Erscheinungsdatum: 20.01.2017
Auflage: 1/2017
Format: 0.5 x 22 x 15
Gewicht: 113 g
Produktform: Kartoniert
Einband: Kartoniert
Artikelnummer: 1211551 Kategorie:

Beschreibung

In this book, a cloned novel modal has been reported that could be transfer to the plants to produce salt tolerant. Current, book includes the retrieval of gene sequence from database and designing of specific primer. Amplification of selected gene with the help of designed primer and cloning of amplified product through TA cloning strategies. Cloned gene was transformed in E. coli DH5 with the help of pRSET-A vector to express the gene. The results showed that the transformed E. coli tolerate up to certain salinity level. Current investigation clearly suggested an unpretentious approach to resolve the problems such as salt stress. This information would be highly useful for the understanding of molecular cloning of genes responsible for salt tolerance in natural environment.

Autorenporträt

Dr. Bablu Prasad is working as an Assistant Professor in the Dept. of Environmental Studies, The M.S. University of Baroda, Vadodara. He has perceived Ph.D. in the area of Environmental Science and Engineering from Indian Institute of Technology Bombay, Mumbai. He has published several research papers in reputed international journals.

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