Genetically Incorporated Non-Canonical Amino Acids
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Methods and Protocols, Methods in Molecular Biology 2676
ISBN: | 1071632507 |
---|---|
ISBN 13: | 9781071632505 |
Herausgeber: | Yu-Hsuan Tsai/Simon J Elsässer |
Verlag: | Springer Verlag GmbH |
Umfang: | xi, 286 S., 1 s/w Illustr., 286 p. 1 illus. |
Erscheinungsdatum: | 06.06.2023 |
Auflage: | 1/2023 |
Produktform: | Gebunden/Hardback |
Einband: | GEB |
This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.
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